For Use by ICCB-L/NSRB Small Molecule and RNAi Screeners

1. Spotfire Decision Site

Spotfire is an interactive data visualization tool that enables screeners to graph very large data sets for the purposes of identifying outlying data points (e.g. hits) and for comparing data sets. This software is only available on the analysis PC in SGM 604. You will need a Harvard Medical School eCommons name and password to log on to the computer.

After clicking on the Spotfire desktop icon, enter (both are case sensitive):
username - Screener
password - ICCBL

A 'Getting Started' box will open that provides an introduction to the software. You can open data files from your directory on an HMS file server, or from a CD or other portable memory device. More details on using Spotfire will be coming.

2. Ingenuity Pathways Analysis

Ingenuity is a biological pathway analysis tool that allows screeners to identify relationships among genes identified in RNAi screens.

You can sign into Ingenuity from any computer that has a Web connection. For information on using Ingenuity, click here to access the HMS Mammalian RNAi Wiki. You will need a Harvard Medical School eCommons username and password for access. For more information on getting access to the RNAi Wiki, click here.

3. Scitegic Pipeline Pilot

Pipeline Pilot can rapidly analyze large datasets according to user-specified computational protocols that are assembled in a modular fashion. Although the program's full functions are available only on workstations at the screening facility, commonly used pipeline protocols may be accessed via a Webport interface.