RNAi Libraries

The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). The below plate numbers are for 384-well plates. Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

Human siRNA Pools

Library Name

Number of siRNA Wells
ICCB Plate Numbers
Dharmacon Human 1: Kinases-Phosphatases 779 50,001-50,003
Dharmacon Human 2: GPCRs 516 50,004-50,005
Dharmacon Human 3: Remaining Druggable Genome 6,022 50,006-50,024
Dharmacon Human 4: Remaining Genome 13,804 50,047-50,090
Mitchison Kinase 1 509 50,091-50,092
Dharmacon Human DUB 1 127 50,439

Mouse siRNA Pools

Library Name
Number of siRNA Wells
ICCB Plate Numbers
Dharmacon Mouse 1: Kinases-Phosphatases 719 50,025-50,027
Dharmacon Mouse 2: GPCRs 474 50,028-50,029
Dharmacon Mouse 3: Remaining Druggable Genome 5,170 50,030-50,046
Dharmacon Mouse 4: Remaining Genome 10,510 50,440-50,472