| Number of compounds |
480 |
| Plate Numbers |
1361-1362
(2 Plates) |
| Concentration Information |
High concentration set is at ~5 mg/ml and
the medium concentration set is at ~1.1 mg/ml (see text below
for details). All compounds are in DMSO. Please denote which
concentration(s) you would like to use when scheduling pin
transfer appointments. |
| Plate Format |
384-well plate
Plate 1361:
Columns 1,24 (empty)
Columns
2,23 (DMSO)
 Click on plate image to enlarge
Plate 1362:
Columns 1,23 (DMSO)
Columns 2, 4, 6,
8, 10, 12, 14, 16, 18, 20, 22, 24 (empty)
 Click on plate image to enlarge
|
This collection was purchased
in April 2004
from BIOMOL (catalogue #2840; www.biomol.com )
and plated in May 2004. It is based on the ICCB-Longwood Bioactives 1 collection (PL-0684-0685). It was assembled by BIOMOL, working together with the ICCB-Longwood, to
provide a library of compounds that affect a wide variety of biological
pathways relevant to ICCB-Longwood screening projects. The collection includes
many classes of compounds
contains
ion channel blockers, GPCR
ligands, second messenger modulators, nuclear receptor ligands,
actin and tubulin ligands, kinase inhibitors, protease inhibitors,
gene regulation agents, lipid biosynthesis inhibitors, as well
as other well-characterized compounds that perturb cell pathways.
We hope that this collection will help both in assay development
and in target identification.
This collection contains 41 FDA-approved drugs. However, investigators wishing to screen the maximum number of FDA-approved drugs can achieve approximately 46% coverage (561 compounds) of the FDA set by screening both NINDS and Prestwick. Including the ICCB Biomol Known Bioactives will increase coverage to ~47% of the FDA set, as there is some redundancy among these three libraries
Because the compounds are biologically active at a variety of
concentrations and because active concentrations differ among cell
types, each compound in the collection is plated at two stock concentrations
on two different plates. Thus there are High and Medium concentration
copies for each plate (see below). We recommend that you screen
the plates at each concentration to get the most information from
your assay:
Copies HA, HB, HC, HD, HF: High concentration plates (approximately
5 mg/ml (~13mM) for most compounds; the high concentration of "potent" compounds
is ~0.5 mg/ml (~1.3mM)).
Copies MA, MB, MC, MD, MF: Medium concentration plates (~1.1 mg/ml
(~3mM); ~0.11 mg/ml (~0.3mM) for "potent" compounds).
Click here to
view a complete list of the compounds in this collection, the concentrations
at which they have been plated, the known activity, and the BIOMOL
catalogue number.
Please see ChemBank for
more details about individual compounds.
|
