ICCB > Screening

This collection was purchased in June 2006 from BIOMOL (catalogue #2840; www.biomol.com ) and plated in October 2006. It is based on the ICCB-Longwood Bioactives 1 collection (PL-0684-0685). This collection is also identical to the compounds that were in the high concentration of plates 1361-1362. It was assembled by BIOMOL, working together with the ICCB-Longwood, to provide a library of compounds that affect a wide variety of biological pathways relevant to ICCB-Longwood screening projects. The collection includes many classes of compounds contains ion channel blockers, GPCR ligands, second messenger modulators, nuclear receptor ligands, actin and tubulin ligands, kinase inhibitors, protease inhibitors, gene regulation agents, lipid biosynthesis inhibitors, as well as other well-characterized compounds that perturb cell pathways. We hope that this collection will help both in assay development and in target identification.

This collection contains 41 FDA-approved drugs. However, investigators wishing to screen the maximum number of FDA-approved drugs can achieve approximately 46% coverage (561 compounds) of the FDA set by screening both NINDS and Prestwick. Including the ICCB Biomol Known Bioactives will increase coverage to ~47% of the FDA set, as there is some redundancy among these three libraries

Because the compounds are biologically active at a variety of concentrations and because active concentrations differ among cell types, each of the compounds are available for screening at two different concentrations. Currently (October 2006) the medium concentration of this library is plated in plates 1361-1362 [medium concentration for each compound is about 1.1 mg/ml (~3mM); or about 0.11 mg/ml (~0.3mM) for "potent" compounds]. For this library, the higher concentration copy of the compounds is plated in Plates 1791-1792 [high concentraion for each compound is about 5 mg/ml (~13mM) for most compounds; or 0.5 mg/ml (~1.3mM) for "potent" compounds]. We recommend that you screen the compounds at each concentration to get the most information from your assay.

Click here to view a complete list of the compounds in this collection, the concentrations at which they have been plated, the known activity, and the BIOMOL catalogue number.