Compound Libraries > Known Bioactives Collection - ICCB Collection 1

The collection was assembled by ICCB-Longwood investigators and collaborators, who chose compounds that affect a wide variety of biological pathways relevant to research projects carried out at the Institute. Most of the compounds are commercially available and include an Ion Channel Ligand library (70 compounds) and a Bioactive Lipids library (200 compounds), both purchased from Biomol, as well as other well-characterized compounds that perturb cell pathways. We hope that this collection will help both in assay development and in target identification.

Because the compounds are biologically active at a variety of concentrations and because active concentrations differ among cell types, each compound in the collection is plated at three stock concentrations on three different plates. Thus there are High, Medium, and Low concentration copies for each plate (see below). We recommend that you screen the plates at all 3 concentrations to get the most information from your assay:

Copies N.O: High concentration plates (approximately 5 mg/ml (~13mM) for most compounds; the high concentration of "potent" compounds is ~0.5 mg/ml (~1.3mM)). All cherry picks will be provided from a high concentration plate.

Copies P.Q: Medium concentration plates (~1.1 mg/ml (~3mM); ~0.11 mg/ml (~0.3mM) for "potent" compounds).

Copies R.S: Low concentration plates (~0.25 mg/ml (~0.65mM); ~0.025 (~65µM)mg/ml for "potent" compounds).

Click here to view a complete list of the compounds in this collection, the concentrations at which they have been plated, the solvents in which they have been diluted, and the suppliers from which they were purchased.

For chemical structures and more information about each compound, please go to the ICCB-Longwood ChemBank site

More details concerning the layout of these plates are in available from our screening database (Activity Base; see the ICCB-Longwood informatics group).